Fluorescence PALM nanoscopy: how does it work, what can it do, and where do we stand ?

Vendredi 06 Janvier 2012 à 11h

Institut de Biologie Structurale J.P. Ebel
41, rue Jules Horowitz
F-38027 GRENOBLE Cedex 1

Résumé:

This seminar will be a progress report about the development of super-resolution fluorescence microscopy carried out in the recently created IBS/iRTSV Pixel team. I will explain the principle of the PALM technique (PhotoActivated Localization Microscopy), which is based on the detection of single photoactivatable fluorescent markers. I will try to give an overview of the recent methodological developments and of the impressive
biological applications that are now emerging in the fields of cell biology, microbiology and neurobiology. Based on the current projects that are presently carried out on-site in collaboration with local teams, I will give some ideas about the challenges that one has to face when embarking into such projects.