SUPER RESOLUTION OPTICAL MICROSCOPY: PRINCIPLES AND APPLICATIONS TO LIFE SCIENCES
In Grenoble, several laboratories share a common interest in engaging or developing super resolution microscopy for life sciences. However, a strong collaboration is necessary to implement the best methods and relevantly match the biological application projects of the local community.
Thus the Nanosciences Foundation organises a preospective workshop on:
Friday 19 June 2009
Conference Room at SPECTRO
Laboratoire de Spectrométrie Physique
St Martin d’Hères Campus, 140 Avenue de la physique - Bat. E45
(http://www-lsp.ujf-grenoble.fr/How-to-find-us?lang=en)
Fluorescence microscopy has become a central tool in life sciences. However, the use of radiation in the visible range, with excitation or emission wavelengths in the micrometer range, limits the achievable spatial resolution to about 0.2 µm (due to diffraction laws), which is more than 10 times the size of macromolecular complexes.
Recently a real revolution has occurred: by using non-linear properties of fluorescence probes, the theoretical diffraction-based spatial resolution limit has been surpassed, leading to “super resolution” or so-called “nanoscopy” methods. Convincing applications in biological sciences have been realized through several approaches, including: STED microscopy, which takes advantage of stimulated emission to spatially confine fluorescence; PALM/STORM/SPDM microscopies, where photoactivatable chromophores
are used to provide super-localization; or SIM microscopy, which is based on a spatial modulation of the illumination field.
This workshop aims at discussing these questions in the context of the Grenoble research.
In order to evaluate the international state-of-the-art of super resolution microscopy techniques, and to steer the debate, we have invited four world-class scientists who have contributed decisively to the field.
- Stefan Jakobs (Max Planck Institute,Göttingen - Germany) is one of the first investigators of the STED method.
- Mark Neil (Imperial College, London – UK) who has invented the structured illumination microscopy
- Heinrich Leonhardt (LMU Biozentrum, Munich - Germany) has published important illustrations of the efficiency of the structured illumination method for cell microscopy.
- Ulrich Nienhaus (University of Karlsruhe - Germany) is a specialist of fluorescent probes highly adapted to the PALM and STORM methodologies.
Stefan Jakobs will give a lecture during an introductory seminar, the day before the workshop (Thursday 18 June 2009). Read about the Seminar
Finalised Workshop program (Download):
8.30am : Coffee
9am : Guests’ lectures and discussion :
Mark Neil: Structured Illumination for 3D microscopy Read the abstract
- Heinrich Leonhardt: Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy Read the abstract
11am : Local scientists’ input
André Verdel (IAB) « Heterochromatin formation and maintenance »
Annie Mola (IAB) « Le complexe passager : régulateur clé de la mitose »
Claire Monge (LBFA) « Intracellular diffusion and organization of metabolism: system biology approach »
Isabelle Marty (GIN) « Molecular complex involved in calcium signaling »
Yves Goldberg (GIN) « Cell-cell communication in the brain via exosome transfer » (PI:R. Sadoul)
Sergiy Avilov (EMBL) « Potential of super-resolution techniques for imaging influenza virus life cycle »
12pm : Countryside lunch
2pm : Guest lecture and discussion :
Ulrich Nienhaus: Advanced Fluorescent Proteins for Optical Nanoscopy Read the abstract
3pm :Local scientists’ input
- Yves Goldberg (GIN) « CHMP2B assemblies and their role in dendritic spine morphogenesis »
- Dimitrios A. Skoufias (IBS) « The need of high resolution microscopy for studies on kinetochore and centromere associated proteins »
- Andrei Popov (GIN) « Action at the microtubule ends »
- Sébastien Violot (iRTSV) « Interaction of actin-binding proteins with actin filaments»
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Sylva Mache (iRTSV) «Single cell transcript profiling and intracellular localisation of transcriptional components»
- Gilles Faury (iRTSV) « Evolution de la morphologie des fibres élastiques au cours du développement et du vieillissement»
Table Ronde : What projects could be promoted in Grenoble by 2010 ?
Laurent BLANCHOIN
Dominique BOURGEOIS
Scientific Committee:
Jacques DEROUARD
Michel ROBERT-NICOUD
Yves GOLDBERG
Andrei POPOV
Alexei GRICHINE
Franz BRUCKERT
Jean-Michel GERARD
Irène WANG
Organisation :
Photo Credits:
Science (journal) 320 (5881): 1332–6. DOI:10.1126/science.1156947. PMID 18535242
Image extracted from http://www.en.wikipedia.org/wiki/Microscopy
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